000187431 001__ 187431
000187431 005__ 20230212173558.0
000187431 0247_ $$2CORDIS$$aG:(EU-Grant)702601$$d702601
000187431 0247_ $$2CORDIS$$aG:(EU-Call)H2020-MSCA-IF-2015$$dH2020-MSCA-IF-2015
000187431 0247_ $$2originalID$$acorda__h2020::702601
000187431 035__ $$aG:(EU-Grant)702601
000187431 150__ $$aGenome editing for spatiotemporal analysis of centriolar SATellite BIOgenesis and FUNction in cellular stress responses$$y2016-07-01 - 2019-06-30
000187431 371__ $$aNational University of Ireland Galway$$bNUI GALWAY$$dIreland$$ehttp://www.nuigalway.ie/$$vCORDIS
000187431 372__ $$aH2020-MSCA-IF-2015$$s2016-07-01$$t2019-06-30
000187431 450__ $$aSATBIOFUN$$wd$$y2016-07-01 - 2019-06-30
000187431 5101_ $$0I:(DE-588b)5098525-5$$2CORDIS$$aEuropean Union
000187431 680__ $$aThe centrosome is the major microtubule-organizing centre of a cell. It also serves as the base for the primary cilium, an antenna-like structure that allows cells to respond to external stimuli. Around the centrosome lie a number of electron dense cytoplasmic granules, termed centriolar satellites. These are involved in protein trafficking and are crucial for normal centrosome and cilium assembly and function. However, little is known about satellite composition, biogenesis or regulation. Mutations in a number of genes encoding satellite proteins cause ciliopathies, demonstrating their importance in the etiology of disease. Emerging data also shows that satellites contribute to the cellular stress response. The central scientific goal of this proposal is to systematically dissect, for the first time, the molecular mechanisms underpinning centriolar satellite biogenesis and function. Coupling genome-editing and cutting-edge imaging techniques we will perform morphometric quantitative analysis of satellite proteins to delineate their specific role in the cellular response to stress. This project encompasses an Outgoing Phase during which the Fellow will be trained in advanced, high-content, automated, and super-resolution microscopy, and computational analysis to study satellite biogenesis and function. Within this phase a secondment to the biotech sector will occur during which the Fellow will learn cutting-edge DNA manipulation methods. The Return Phase allows the transfer of this knowledge to Europe whilst the Fellow receives training in the analysis of stress responses. Alongside this the Fellow will follow a program of courses to acquire a range of complementary skills, including industry-related skills and teaching qualifications. Together, this will accomplish the overarching objective of developing the Fellow’s career to a position of professional maturity, thereby facilitating her long-term goal of establishing her own independent research group in Europe.
000187431 909CO $$ooai:juser.fz-juelich.de:506544$$pauthority$$pauthority:GRANT
000187431 909CO $$ooai:juser.fz-juelich.de:506544
000187431 970__ $$aoai:dnet:corda__h2020::daa6762111f9ebf62b9c2ec655512783
000187431 980__ $$aG
000187431 980__ $$aCORDIS
000187431 980__ $$aAUTHORITY