Record #188418 - GSI Repository

Glycoli

Design of glycan epitope toolbox: a novel pathway for protein N-glycosylation in the E. coli cytosol

Coordinator	Swiss Federal Institute of Technology in Zurich
Grant period	2016-11-01 - 2018-10-31
Funding body	European Union
Call number	H2020-MSCA-IF-2015
Grant number	703577
Identifier	G:(EU-Grant)703577

Note: Metabolic engineering of E. coli has been highly successful in producing diverse free oligosaccharides for research and commercial applications. Currently, we are poised to address the next major challenge: the site-specific synthesis of oligosaccharides directly onto proteins in the E. coli cytosol. In this project we aim at the metabolic engineering of an N-glycosylation pathway in the E. coli cytosol using heterologous glycosyltransferases (GTs) to produce defined glycoprotein structures. Starting point is the newly discovered family of cytosolic N-glycosyltransferases (NGT), which transfer a single glucose residue onto proteins at asparagine (N) residues in the N-X-S/T sequon. Co-expression of an NGT with a galactosyltransferase has been shown to yield N-linked lactose in the E. coli cytosol. This N-linked lactose is an ideal starting point for the design of cytosolic N-glycosylation pathways to synthesize important glycans on proteins. Two goals are delineated. 1) Bacterial GTs will be screened, and potentially engineered, for their ability to extend the N-lactose with defined sugars, thereby generating a diverse repertoire of glycans directly synthesized on proteins. The focus will be on screening of fucosyltransferases and efficient production of fucosylated oligosaccharides, as these are central glycan epitopes in diverse physiological processes. 2) The newly developed glycosylation pathways will be applied to a range of protein substrates in order to explore and address possible limitations of the glycosylation system. The result will be a well-characterized glycoengineering toolbox enabling the bottom-up production of defined glycoprotein structures in E. coli.

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Record created 2016-06-03, last modified 2023-02-08

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