000204796 001__ 204796
000204796 005__ 20230208173709.0
000204796 0247_ $$2CORDIS$$aG:(EU-Grant)737560$$d737560
000204796 0247_ $$2CORDIS$$aG:(EU-Call)ERC-2016-PoC$$dERC-2016-PoC
000204796 0247_ $$2originalID$$acorda__h2020::737560
000204796 035__ $$aG:(EU-Grant)737560
000204796 150__ $$aDevelopment of high-resolution, high-throughput microscopy$$y2017-03-01 - 2018-08-31
000204796 371__ $$aInserm$$bINSERM$$dFrance$$ehttp://english.inserm.fr/$$vCORDIS
000204796 372__ $$aERC-2016-PoC$$s2017-03-01$$t2018-08-31
000204796 450__ $$aInstantScope$$wd$$y2017-03-01 - 2018-08-31
000204796 5101_ $$0I:(DE-588b)5098525-5$$2CORDIS$$aEuropean Union
000204796 680__ $$aCurrent 3D microscopy methods are limited in speed due to the need to scan the sample in the optical axis. Multifocus microscopy - or MFM- is a method that allows for the instant acquisition of a 3D volume without scanning, thus increasing acquisition speed by a factor of 10-100. MFM based on diffractive optics allows for easy optimization of the total volume acquired and the sampling rate. In our ERC-StG project, we developed an important expertise in the design and construction of diffractive elements key to MFM and invented a novel super-resolution imaging method based on this technology (patent pending). In this project, we propose to further improve the conception and fabrication methods of MFM’s diffractive elements to considerably improve acquisition speed and resolution. In addition, we will employ these technologies in conjunction with valve-based microfluidics to develop and validate a microscopy-based high-resolution, high-throughput cell profiler (Hi2M). Hi2M will allow for the 3D acquisition of complete cell volumes in <10 ms, enabling the high-resolution, multi-color imaging of millions of cells/hour without the need for cell adhesion. Because of these important advantages over existing microscopies, we anticipate that the technologies developed in InstantScope should have a large impact in many fields of cell biology.
000204796 909CO $$ooai:juser.fz-juelich.de:833573$$pauthority$$pauthority:GRANT
000204796 909CO $$ooai:juser.fz-juelich.de:833573
000204796 970__ $$aoai:dnet:corda__h2020::15db41c6c374d753233bd5e37a4da837
000204796 980__ $$aG
000204796 980__ $$aCORDIS
000204796 980__ $$aAUTHORITY