000234719 001__ 234719
000234719 005__ 20201020173029.0
000234719 0247_ $$2I:(DE-H235)DIB-20120731$$aG:(DE-HGF)2020_VH-NG-1502$$dVH-NG-1502
000234719 035__ $$aG:(DE-HGF)2020_VH-NG-1502
000234719 150__ $$aRole of Enzyme Dynamics in Catalysis Revealed by Time Resolved Crystallography$$y2020-2026
000234719 371__ $$0P:(DE-H253)PIP1020283$$aLane, Thomas
000234719 371__ $$aPearson, Arwen
000234719 450__ $$aVH-NG-1502$$wd$$y2020-2026
000234719 5101_ $$0I:(DE-588b)5165524-X$$aHelmholtz Gemeinschaft Deutscher Forschungszentren$$bHGF
000234719 550__ $$0G:(DE-HGF)IVF-20140101$$aImpuls- und Vernetzungsfonds$$wt
000234719 680__ $$aEnzymes are biology’s machines for doing chemistry. In addition to being central to life as we know it, they often perform desirable reactions many orders of magnitude faster than man-made catalysts with stereoselectivity and specificity. Yet a full physical description of how this is possible is lacking. As a result, we do not fully understand life and our ability to create new enzymes is limited. I propose to address this knowledge gap by imaging enzymes while they perform catalysis. Since chemistry involves the rearrangement of bonds, imaging of such processes requires atomic spatial resolution and femtosecond temporal resolution, only recently possible thanks to X-ray free electron lasers like the European XFEL. Such experiments require initiation of the enzyme reaction with femtosecond temporal resolution, currently only possible using ultrafast lasers. Therefore, we will first study one of the few naturally light-triggered enzymes, DNA photolyase, which conducts a DNA repair reaction. Simultaneously, we will pursue the development of a light-activated enzyme cofactor, a photoNAD(P)H. This will enable us to turn the drug target DHFR into a light-activated system, allowing us to image it, resolving open questions about how it functions while demonstrating the new photoNAD(P)H technology.
000234719 8564_ $$uhttps://hgf.desy.de/ivf/projekte/e307615/index_ger.html
000234719 909CO $$ooai:juser.fz-juelich.de:885774$$pauthority:GRANT$$pauthority
000234719 909CO $$ooai:juser.fz-juelich.de:885774
000234719 980__ $$aG
000234719 980__ $$aAUTHORITY