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The extracellular matrix of perineuronal nets: Dynamics of molecular organization and functional analysis
Note: Perin Euro Nale networks (PNs) of the extracellular matrix including specifically influence the properties of the extracellular space on the surface of many types of CNS neurons, thereby presynaptic boutons and neighboring Gliazellfortsätze including. Our previous studies and the work of other groups have shown that Chondroitinsulfatproteoglykane the aggrecan family form the glycosaminoglycan hyaluronic acid and the glycoproteins tenascin-R and -C variable main components of PNs. Tenascin-R plays an important role in the formation of macromolecular complexes during brain development and for the preservation of the integrity of the extracellular matrix in the adult state. The aim of the proposed project is to elucidate the functions of the PNs that were previously mainly the stabilization of synapses and the support of neuronal activity associated with hypothetical. Here are three aspects are: 1) mechanisms of training and the restoration of PNs by the various matrix components. The role of neuronal and glial matrix receptors is analyzed and identified for training of PNs essential cell surface molecules. We intend to reconstruct PNs heterologous by transfection in vitro cells defined with different matrix receptors and adding matrix components. 2) Functional properties of native and reconstituted PNs. Parvalbumin-expressing interneurons, which are covered in the rule of PNs should be visualized by the green-by transgenic expression fluorescent protein (GFP). Your discharge patterns, K + - and Ca2 + currents and the inhibitory and excitatory synaptic inputs are then electrophysiology, immunocytochemistry and characterized by Vital mark active synapses. Enzyme treatments destroy the PNs and tenascin-R-deficient mice with altered PNs are part of these experiments. Similar parameters are studied in heterologous neural cells that were transfected with individual proteins necessary for PN formation. 3) Depending on the postnatal training and the reorganization of PNs in the adult condition of an intact sensory input. These in vivo experiments are on somatosensory and visual cortex of mice with established models (Vibrissektomie and visual deprivation) performed. Moreover, to be examined whether tenascin-R deficiency to changes in training sensory cortical maps, including PNs involved in inhibitory circuits leads.
| Grant period | 2000-01-01 - 2007-12-31 |
| Funding body | German Research Foundation |
| DFG | |
| Grant number | grant.4752803 |
| Identifier | G:(GEPRIS)5223744 |
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Record created 2021-07-01, last modified 2021-09-24