000242874 001__ 242874
000242874 005__ 20230214173927.0
000242874 0247_ $$2CORDIS$$aG:(EU-Grant)101031624$$d101031624
000242874 0247_ $$2CORDIS$$aG:(EU-Call)H2020-MSCA-IF-2020$$dH2020-MSCA-IF-2020
000242874 0247_ $$2originalID$$acorda__h2020::101031624
000242874 035__ $$aG:(EU-Grant)101031624
000242874 150__ $$aDeciphering collagen mineralization process by dynamic imaging in liquid$$y2021-04-01 - 2023-03-31
000242874 372__ $$aH2020-MSCA-IF-2020$$s2021-04-01$$t2023-03-31
000242874 450__ $$aDYNAMIN$$wd$$y2021-04-01 - 2023-03-31
000242874 5101_ $$0I:(DE-588b)5098525-5$$2CORDIS$$aEuropean Union
000242874 680__ $$aBone is a complex nanocomposite with outstanding mechanical properties arising from the nanoscale interaction between its two main building blocks: type I collagen fibrils and hydroxyapatite crystals. Despite its clinical relevance, the mechanisms governing bone formation are still poorly understood, mainly due to the complexity of the processes. During bone formation and remodeling, non-collagenous proteins and proteoglycans act synergistically to regulate the mineral deposition process, participating in multiple signaling pathways involving regulatory molecules, osteoblasts and osteoclasts.
Many of the studies performed until now relayed into simplified in vitro models that hardly represent this complexity. Furthermore, methods traditionally applied only provide snapshots of this process, unable to extract dynamic information. To really understand the mechanisms regulating bone mineralization, we need to simultaneously visualize its different components in its context, to be able to monitor their interactions. 
In this project, I propose to combining liquid-phase electron microscopy and immunolabelling, with which I aim to bring together dynamic imaging of a complex biochemical process and the identification of the biomacromolecules involved. By recreating the mineralization conditions inside the liquid cell, I aim to obtain real time data on nucleation sites, crystal growth and mineralization dynamics. The combination of dynamic imaging with immunogold will allow me to monitor the direct interaction of these regulatory molecules with the mineral particles at nanoscale resolution. By means of this new exciting approach I expect to provide unprecedented data on the processes of bone formation and take a step forward in the application of LPEM in biological materials.
000242874 909CO $$ooai:juser.fz-juelich.de:899314$$pauthority$$pauthority:GRANT
000242874 909CO $$ooai:juser.fz-juelich.de:899314
000242874 980__ $$aG
000242874 980__ $$aCORDIS
000242874 980__ $$aAUTHORITY