000244945 001__ 244945
000244945 005__ 20230208174202.0
000244945 0247_ $$2CORDIS$$aG:(EU-Grant)892252$$d892252
000244945 0247_ $$2CORDIS$$aG:(EU-Call)H2020-MSCA-IF-2019$$dH2020-MSCA-IF-2019
000244945 0247_ $$2originalID$$acorda__h2020::892252
000244945 035__ $$aG:(EU-Grant)892252
000244945 150__ $$aCharacterisation of permissive and non-permissive phagosome environments during Salmonella systemic infection$$y2021-09-01 - 2023-08-31
000244945 372__ $$aH2020-MSCA-IF-2019$$s2021-09-01$$t2023-08-31
000244945 450__ $$aSalPhagoHet$$wd$$y2021-09-01 - 2023-08-31
000244945 5101_ $$0I:(DE-588b)5098525-5$$2CORDIS$$aEuropean Union
000244945 680__ $$aInfectious diseases represent a major threat to global human health today. Antibiotic misuse has induced rapid emergence of resistant bacteria and it is becoming increasingly challenging to identify new, efficient drugs. Novel control strategies are urgently required. This project intends to uncover key host immune factors determining the outcome of a bacterial infection with the aim to identify strategies to synergise with the immune system. Emerging evidence suggests that heterogeneous host-pathogen encounters play a key role in disease outcome. To investigate the role of distinct tissue microenvironments during infection, I will use Salmonella enterica to study the immune properties of neutrophils and resident tissue macrophages, previously shown to be non-permissive and permissive environments, respectively. Specifically, I will use a novel approach of phagosome isolation by fluorescence-activated cell sorting and will combine it with sensitive high-resolution mass spectrometry. With this state-of-the-art technology, I will identify key host factors required for phagosome formation by proteomics, which will provide relevant insights into the biology of two key professional phagocytes with important applications in infection biology and immunology. Secondly, I will analyse Salmonella responses to these host determinants using proteomics and stress-inducible promoter fusions to reporter genes. This will highlight key bacterial strategies for intracellular survival and thus novel antimicrobial targets. Finally, I will confirm the in vivo role of identified key factors using a murine infection model. This integrated approach, investigating the host and the pathogen, will be broadly applicable to study other important infectious diseases. This project will highlight specific host and pathogen responses with the aim to close permissive niches where suboptimal antimicrobial exposure allows pathogens to survive and spread.
000244945 909CO $$ooai:juser.fz-juelich.de:901385$$pauthority$$pauthority:GRANT
000244945 909CO $$ooai:juser.fz-juelich.de:901385
000244945 980__ $$aG
000244945 980__ $$aCORDIS
000244945 980__ $$aAUTHORITY