000245249 001__ 245249
000245249 005__ 20230208174206.0
000245249 0247_ $$2CORDIS$$aG:(EU-Grant)894574$$d894574
000245249 0247_ $$2CORDIS$$aG:(EU-Call)H2020-MSCA-IF-2019$$dH2020-MSCA-IF-2019
000245249 0247_ $$2originalID$$acorda__h2020::894574
000245249 035__ $$aG:(EU-Grant)894574
000245249 150__ $$aGene network rewiring by an engineered CRIPSR-Cas12a variant$$y2020-09-01 - 2022-08-31
000245249 372__ $$aH2020-MSCA-IF-2019$$s2020-09-01$$t2022-08-31
000245249 450__ $$aGNRECC$$wd$$y2020-09-01 - 2022-08-31
000245249 5101_ $$0I:(DE-588b)5098525-5$$2CORDIS$$aEuropean Union
000245249 680__ $$aIn the last 10 years, an increasing number of studies employed -omics technologies to describe the molecular changes underpinning cellular functions. However, a large part of these findings is awaiting an experimental validation. This is due to the lack of efficient molecular tools able to control both multiple and distinct genetic interactions.  Recently, I described a new CRISPR/12-based genome engineering tool that, for the first time, it provides the constitutive, conditional, inducible, orthogonal and multiplexed engineering of dozens of endogenous genes, simultaneously. In this research proposal, I aim to increase the efficiency of this platform in the context of multiplexed genome engineering applications, such as gene network rewiring. To this aim, I will use techniques inspired by the statistical physics of complex disordered systems, to design a more potent version of my CRISPR/Cas12-based genome engineering tool and, I will use this novel platform to rewire signaling pathways involved in cellular proliferation. This research proposal is structured into the following tasks: rational design of novel Cas12a variants, validation of novel Cas12a variants, gene network rewiring by a novel Cas12a variant.  By coupling statistical physics to both protein and genome engineering this project will pave the way for efficient and large-scale engineering of gene networks.
000245249 909CO $$ooai:juser.fz-juelich.de:901689$$pauthority$$pauthority:GRANT
000245249 909CO $$ooai:juser.fz-juelich.de:901689
000245249 980__ $$aG
000245249 980__ $$aCORDIS
000245249 980__ $$aAUTHORITY