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| 001 | 245249 | ||
| 005 | 20230208174206.0 | ||
| 024 | 7 | _ | |a G:(EU-Grant)894574 |d 894574 |2 CORDIS |
| 024 | 7 | _ | |a G:(EU-Call)H2020-MSCA-IF-2019 |d H2020-MSCA-IF-2019 |2 CORDIS |
| 024 | 7 | _ | |a corda__h2020::894574 |2 originalID |
| 035 | _ | _ | |a G:(EU-Grant)894574 |
| 150 | _ | _ | |a Gene network rewiring by an engineered CRIPSR-Cas12a variant |y 2020-09-01 - 2022-08-31 |
| 372 | _ | _ | |a H2020-MSCA-IF-2019 |s 2020-09-01 |t 2022-08-31 |
| 450 | _ | _ | |a GNRECC |w d |y 2020-09-01 - 2022-08-31 |
| 510 | 1 | _ | |0 I:(DE-588b)5098525-5 |a European Union |2 CORDIS |
| 680 | _ | _ | |a In the last 10 years, an increasing number of studies employed -omics technologies to describe the molecular changes underpinning cellular functions. However, a large part of these findings is awaiting an experimental validation. This is due to the lack of efficient molecular tools able to control both multiple and distinct genetic interactions. Recently, I described a new CRISPR/12-based genome engineering tool that, for the first time, it provides the constitutive, conditional, inducible, orthogonal and multiplexed engineering of dozens of endogenous genes, simultaneously. In this research proposal, I aim to increase the efficiency of this platform in the context of multiplexed genome engineering applications, such as gene network rewiring. To this aim, I will use techniques inspired by the statistical physics of complex disordered systems, to design a more potent version of my CRISPR/Cas12-based genome engineering tool and, I will use this novel platform to rewire signaling pathways involved in cellular proliferation. This research proposal is structured into the following tasks: rational design of novel Cas12a variants, validation of novel Cas12a variants, gene network rewiring by a novel Cas12a variant. By coupling statistical physics to both protein and genome engineering this project will pave the way for efficient and large-scale engineering of gene networks. |
| 909 | C | O | |o oai:juser.fz-juelich.de:901689 |p authority:GRANT |p authority |
| 909 | C | O | |o oai:juser.fz-juelich.de:901689 |
| 980 | _ | _ | |a G |
| 980 | _ | _ | |a CORDIS |
| 980 | _ | _ | |a AUTHORITY |
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