000343870 001__ 343870
000343870 005__ 20230827173441.0
000343870 0247_ $$2CORDIS$$aG:(EU-Grant)101130807$$d101130807
000343870 0247_ $$2CORDIS$$aG:(EU-Call)HORIZON-WIDERA-2022-TALENTS-04$$dHORIZON-WIDERA-2022-TALENTS-04
000343870 0247_ $$2originalID$$acorda_____he::101130807
000343870 035__ $$aG:(EU-Grant)101130807
000343870 150__ $$aO-Glc-NAc modifications of RNA-binding proteins$$y2023-07-01 - 2025-08-31
000343870 372__ $$aHORIZON-WIDERA-2022-TALENTS-04$$s2023-07-01$$t2025-08-31
000343870 450__ $$aOMRP$$wd$$y2023-07-01 - 2025-08-31
000343870 5101_ $$0I:(DE-588b)5098525-5$$2CORDIS$$aEuropean Union
000343870 680__ $$aO-GlcNAcylation is a post-translational dynamic modification of proteins that is becoming more widely recognized as a modification that can protect proteins from pathological aggregation in neurodegenerative diseases. However, it is unknown how this modification influences the RNA binding properties of the protein and its function. In the proposed project I will study the O-Glc-NAcylation of RNA-binding proteins (RBPs) associated with amyotrophic lateral sclerosis (ALS), and its effects on the RNA binding properties and thereby on the regulation of RNA processing. I will find O-GlcNAcylation sites of TDP-43, Fused-in-sarcoma (FUS), Ewing sarcoma protein (EWS), and TATA-box binding protein associated factor 15 (TAF15) and I will assess the increased amounts of modification after stimulation of the cells with high glucose or with O-Glc-NAcase inhibitor. I will study if their RNA interaction profiles change with increasing amounts of O-Glc-NAc modifications in differentiated cortical neurons. Later, I plan to implement in the company, a mass spectrometry method adapted to analyse the ALS-liked RBPs proteins, which will help the glycoscience community. This project will expand our understanding of the molecular mechanism of transcription regulation through protein O-Glc-NAcylation and can provide the necessary knowledge to understand ALS-related proteinopathies.
000343870 909CO $$ooai:juser.fz-juelich.de:1013230$$pauthority:GRANT$$pauthority
000343870 909CO $$ooai:juser.fz-juelich.de:1013230
000343870 980__ $$aG
000343870 980__ $$aCORDIS
000343870 980__ $$aAUTHORITY