000343875 001__ 343875
000343875 005__ 20230827173442.0
000343875 0247_ $$2CORDIS$$aG:(EU-Grant)101095380$$d101095380
000343875 0247_ $$2CORDIS$$aG:(EU-Call)ERC-2022-ADG$$dERC-2022-ADG
000343875 0247_ $$2originalID$$acorda_____he::101095380
000343875 035__ $$aG:(EU-Grant)101095380
000343875 150__ $$aHow plant cells set the tempo of rhythmic shoot construction$$y2024-01-01 - 2028-12-31
000343875 372__ $$aERC-2022-ADG$$s2024-01-01$$t2028-12-31
000343875 450__ $$aTEMPO$$wd$$y2024-01-01 - 2028-12-31
000343875 5101_ $$0I:(DE-588b)5098525-5$$2CORDIS$$aEuropean Union
000343875 680__ $$aTime is central to the development of the body plan of multicellular organisms. One prominent developmental timing mechanism is the rhythmic, iterative addition of tissues and organs. While the tempo of rhythmic construction is often set by developmental clocks, plants use a unique mechanism in the shoot, whereby rhythmic organogenesis emerges from dynamic changes in the distribution of the hormone auxin. High auxin levels trigger organogenesis but, contrary to a long-standing theory, the period of shoot organ production - or plastochron - cannot simply be encoded in periodic auxin oscillations, due to noise in these oscillations. Revealing how the tempo of shoot construction is established thus remains a critical knowledge gap in plant biology.
In TEMPO, we hypothesize that cells record and use the history of their auxin exposure, in order to robustly set the timing of organogenesis and the plastochron at the tissue scale despite noisy auxin temporal information. This fundamental change in the way we understand the relationship between auxin and the plastochron stems from preliminary data from my team suggesting histone acetylation as an epigenetic-tracking mechanism, which allows auxin temporal information to be recorded and utilized for transcriptional control.
Uncovering how auxin temporal information establishes the tempo of shoot construction requires multiscale, multidisciplinary approaches. We will combine cutting-edge live-imaging, synthetic biology and computational modeling with innovative optogenetics and single-cell genomics to both ascertain and perturb auxin temporal information and histone acetylation at high resolution, while assessing the effect on cellular transcriptional states and the timing of organ production. Beyond testing whether epigenetic tracking of auxin temporal information sets a robust plastochron across scales, we will reengineer the plastochron to demonstrate that the tempo of shoot construction can be predictively manipulated.
000343875 909CO $$ooai:juser.fz-juelich.de:1013235$$pauthority:GRANT$$pauthority
000343875 909CO $$ooai:juser.fz-juelich.de:1013235
000343875 980__ $$aG
000343875 980__ $$aCORDIS
000343875 980__ $$aAUTHORITY