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000354897 1001_ $$0P:(DE-HGF)0$$aRadstake, Wilhelmina E.$$b0
000354897 245__ $$aRadiation-induced DNA double-strand breaks in cortisol exposed fibroblasts as quantified with the novel foci-integrated damage complexity score (FIDCS)
000354897 260__ $$a[London]$$bMacmillan Publishers Limited, part of Springer Nature$$c2024
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000354897 500__ $$aGrant: SA-IBER (AO-2019-IBER) " This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/."
000354897 520__ $$aWithout the protective shielding of Earth's atmosphere, astronauts face higher doses of ionizing radiation in space, causing serious health concerns. Highly charged and high energy (HZE) particles are particularly effective in causing complex and difficult-to-repair DNA double-strand breaks compared to low linear energy transfer. Additionally, chronic cortisol exposure during spaceflight raises further concerns, although its specific impact on DNA damage and repair remains unknown. This study explorers the effect of different radiation qualities (photons, protons, carbon, and iron ions) on the DNA damage and repair of cortisol-conditioned primary human dermal fibroblasts. Besides, we introduce a new measure, the Foci-Integrated Damage Complexity Score (FIDCS), to assess DNA damage complexity by analyzing focus area and fluorescent intensity. Our results show that the FIDCS captured the DNA damage induced by different radiation qualities better than counting the number of foci, as traditionally done. Besides, using this measure, we were able to identify differences in DNA damage between cortisol-exposed cells and controls. This suggests that, besides measuring the total number of foci, considering the complexity of the DNA damage by means of the FIDCS can provide additional and, in our case, improved information when comparing different radiation qualities.
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000354897 588__ $$aDataset connected to CrossRef, Journals: repository.gsi.de
000354897 650_7 $$2Other$$aCarbon ions
000354897 650_7 $$2Other$$aCortisol
000354897 650_7 $$2Other$$aDNA damage
000354897 650_7 $$2Other$$aDNA repair
000354897 650_7 $$2Other$$aFibroblast
000354897 650_7 $$2Other$$aIonizing radiation
000354897 650_7 $$2Other$$aIron ions
000354897 650_7 $$2Other$$aProtons
000354897 650_7 $$0WI4X0X7BPJ$$2NLM Chemicals$$aHydrocortisone
000354897 650_2 $$2MeSH$$aHumans
000354897 650_2 $$2MeSH$$aFibroblasts: radiation effects
000354897 650_2 $$2MeSH$$aFibroblasts: metabolism
000354897 650_2 $$2MeSH$$aDNA Breaks, Double-Stranded: radiation effects
000354897 650_2 $$2MeSH$$aHydrocortisone: pharmacology
000354897 650_2 $$2MeSH$$aDNA Repair
000354897 650_2 $$2MeSH$$aRadiation, Ionizing
000354897 650_2 $$2MeSH$$aCells, Cultured
000354897 650_2 $$2MeSH$$aDNA Damage
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000354897 7001_ $$0P:(DE-HGF)0$$aParisi, Alessio$$b1
000354897 7001_ $$0P:(DE-HGF)0$$aMiranda, Silvana$$b2
000354897 7001_ $$0P:(DE-HGF)0$$aGautam, Kiran$$b3
000354897 7001_ $$0P:(DE-HGF)0$$aVermeesen, Randy$$b4
000354897 7001_ $$0P:(DE-HGF)0$$aRehnberg, Emil$$b5
000354897 7001_ $$0P:(DE-HGF)0$$aTabury, Kevin$$b6
000354897 7001_ $$0P:(DE-HGF)0$$aCoppes, Rob$$b7
000354897 7001_ $$0P:(DE-HGF)0$$avan Goethem, Marc-Jan$$b8
000354897 7001_ $$0P:(DE-HGF)0$$aBrandenburg, Sytze$$b9
000354897 7001_ $$0P:(DE-Ds200)OR2545$$aWeber, Ulrich$$b10
000354897 7001_ $$0P:(DE-Ds200)OR0311$$aFournier, Claudia$$b11
000354897 7001_ $$0P:(DE-Ds200)OR2413$$aDurante, Marco$$b12
000354897 7001_ $$0P:(DE-HGF)0$$aBaselet, Bjorn$$b13$$eCorresponding author
000354897 7001_ $$0P:(DE-HGF)0$$aBaatout, Sarah$$b14
000354897 773__ $$0PERI:(DE-600)2615211-3$$a10.1038/s41598-024-60912-y$$gVol. 14, no. 1, p. 10400$$n1$$p10400$$tScientific reports$$v14$$x2045-2322$$y2024
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