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@ARTICLE{Radstake:354897,
      author       = {Radstake, Wilhelmina E. and Parisi, Alessio and Miranda,
                      Silvana and Gautam, Kiran and Vermeesen, Randy and Rehnberg,
                      Emil and Tabury, Kevin and Coppes, Rob and van Goethem,
                      Marc-Jan and Brandenburg, Sytze and Weber, Ulrich and
                      Fournier, Claudia and Durante, Marco and Baselet, Bjorn and
                      Baatout, Sarah},
      title        = {{R}adiation-induced {DNA} double-strand breaks in cortisol
                      exposed fibroblasts as quantified with the novel
                      foci-integrated damage complexity score ({FIDCS})},
      journal      = {Scientific reports},
      volume       = {14},
      number       = {1},
      issn         = {2045-2322},
      address      = {[London]},
      publisher    = {Macmillan Publishers Limited, part of Springer Nature},
      reportid     = {GSI-2025-00017},
      pages        = {10400},
      year         = {2024},
      note         = {Grant: SA-IBER (AO-2019-IBER) " This article is licensed
                      under a Creative Commons Attribution 4.0 International
                      License, which permits use, sharing, adaptation,
                      distribution and reproduction in any medium or format, as
                      long as you give appropriate credit to the original
                      author(s) and the source, provide a link to the Creative
                      Commons licence, and indicate if changes were made. The
                      images or other third party material in this article are
                      included in the article's Creative Commons licence, unless
                      indicated otherwise in a credit line to the material. If
                      material is not included in the article's Creative Commons
                      licence and your intended use is not permitted by statutory
                      regulation or exceeds the permitted use, you will need to
                      obtain permission directly from the copyright holder. To
                      view a copy of this licence, visit
                      http://creativecommons.org/licenses/by/4.0/."},
      abstract     = {Without the protective shielding of Earth's atmosphere,
                      astronauts face higher doses of ionizing radiation in space,
                      causing serious health concerns. Highly charged and high
                      energy (HZE) particles are particularly effective in causing
                      complex and difficult-to-repair DNA double-strand breaks
                      compared to low linear energy transfer. Additionally,
                      chronic cortisol exposure during spaceflight raises further
                      concerns, although its specific impact on DNA damage and
                      repair remains unknown. This study explorers the effect of
                      different radiation qualities (photons, protons, carbon, and
                      iron ions) on the DNA damage and repair of
                      cortisol-conditioned primary human dermal fibroblasts.
                      Besides, we introduce a new measure, the Foci-Integrated
                      Damage Complexity Score (FIDCS), to assess DNA damage
                      complexity by analyzing focus area and fluorescent
                      intensity. Our results show that the FIDCS captured the DNA
                      damage induced by different radiation qualities better than
                      counting the number of foci, as traditionally done. Besides,
                      using this measure, we were able to identify differences in
                      DNA damage between cortisol-exposed cells and controls. This
                      suggests that, besides measuring the total number of foci,
                      considering the complexity of the DNA damage by means of the
                      FIDCS can provide additional and, in our case, improved
                      information when comparing different radiation qualities.},
      keywords     = {Humans / Fibroblasts: radiation effects / Fibroblasts:
                      metabolism / DNA Breaks, Double-Stranded: radiation effects
                      / Hydrocortisone: pharmacology / DNA Repair / Radiation,
                      Ionizing / Cells, Cultured / DNA Damage / Carbon ions
                      (Other) / Cortisol (Other) / DNA damage (Other) / DNA repair
                      (Other) / Fibroblast (Other) / Ionizing radiation (Other) /
                      Iron ions (Other) / Protons (Other) / Hydrocortisone (NLM
                      Chemicals)},
      cin          = {BIO / BIO@FAIR},
      ddc          = {600},
      cid          = {I:(DE-Ds200)BIO-20160831OR354 / I:(DE-Ds200)Coll-FAIR-BIO},
      pnm          = {633 - Life Sciences – Building Blocks of Life: Structure
                      and Function (POF4-633) / FAIR Phase-0 - FAIR Phase-0
                      Research Program (GSI-FAIR-Phase-0) / SUC-GSI-Darmstadt -
                      Strategic university cooperation GSI-TU Darmstadt
                      (SUC-GSI-DA)},
      pid          = {G:(DE-HGF)POF4-633 / G:(Ds200)GSI-FAIR-Phase-0 /
                      G:(DE-Ds200)SUC-GSI-DA},
      experiment   = {EXP:(DE-Ds200)SESA-20200803},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38710823},
      UT           = {WOS:001253949600063},
      doi          = {10.1038/s41598-024-60912-y},
      url          = {https://repository.gsi.de/record/354897},
}