Radiation-induced DNA double-strand breaks in cortisol exposed fibroblasts as quantified with the novel foci-integrated damage complexity score (FIDCS)

Radstake, Wilhelmina E.; Gautam, Kiran; Vermeesen, Randy; Durante, Marco; Miranda, Silvana; Brandenburg, Sytze; Baatout, Sarah; van Goethem, Marc-Jan; Tabury, Kevin; Rehnberg, Emil; Parisi, Alessio; Coppes, Rob; Fournier, Claudia; Weber, Ulrich; Baselet, Bjorn

doi:10.1038/s41598-024-60912-y

Items
Marc 21

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024	7	_	\|a 10.1038/s41598-024-60912-y \|2 doi
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041	_	_	\|a English
082	_	_	\|a 600
100	1	_	\|a Radstake, Wilhelmina E. \|0 P:(DE-HGF)0 \|b 0
245	_	_	\|a Radiation-induced DNA double-strand breaks in cortisol exposed fibroblasts as quantified with the novel foci-integrated damage complexity score (FIDCS)
260	_	_	\|a [London] \|c 2024 \|b Macmillan Publishers Limited, part of Springer Nature
336	7	_	\|a article \|2 DRIVER
336	7	_	\|a Output Types/Journal article \|2 DataCite
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336	7	_	\|a ARTICLE \|2 BibTeX
336	7	_	\|a JOURNAL_ARTICLE \|2 ORCID
336	7	_	\|a Journal Article \|0 0 \|2 EndNote
500	_	_	\|a Grant: SA-IBER (AO-2019-IBER) " This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/."
520	_	_	\|a Without the protective shielding of Earth's atmosphere, astronauts face higher doses of ionizing radiation in space, causing serious health concerns. Highly charged and high energy (HZE) particles are particularly effective in causing complex and difficult-to-repair DNA double-strand breaks compared to low linear energy transfer. Additionally, chronic cortisol exposure during spaceflight raises further concerns, although its specific impact on DNA damage and repair remains unknown. This study explorers the effect of different radiation qualities (photons, protons, carbon, and iron ions) on the DNA damage and repair of cortisol-conditioned primary human dermal fibroblasts. Besides, we introduce a new measure, the Foci-Integrated Damage Complexity Score (FIDCS), to assess DNA damage complexity by analyzing focus area and fluorescent intensity. Our results show that the FIDCS captured the DNA damage induced by different radiation qualities better than counting the number of foci, as traditionally done. Besides, using this measure, we were able to identify differences in DNA damage between cortisol-exposed cells and controls. This suggests that, besides measuring the total number of foci, considering the complexity of the DNA damage by means of the FIDCS can provide additional and, in our case, improved information when comparing different radiation qualities.
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588	_	_	\|a Dataset connected to CrossRef, Journals: repository.gsi.de
650	_	7	\|a Carbon ions \|2 Other
650	_	7	\|a Cortisol \|2 Other
650	_	7	\|a DNA damage \|2 Other
650	_	7	\|a DNA repair \|2 Other
650	_	7	\|a Fibroblast \|2 Other
650	_	7	\|a Ionizing radiation \|2 Other
650	_	7	\|a Iron ions \|2 Other
650	_	7	\|a Protons \|2 Other
650	_	7	\|a Hydrocortisone \|0 WI4X0X7BPJ \|2 NLM Chemicals
650	_	2	\|a Humans \|2 MeSH
650	_	2	\|a Fibroblasts: radiation effects \|2 MeSH
650	_	2	\|a Fibroblasts: metabolism \|2 MeSH
650	_	2	\|a DNA Breaks, Double-Stranded: radiation effects \|2 MeSH
650	_	2	\|a Hydrocortisone: pharmacology \|2 MeSH
650	_	2	\|a DNA Repair \|2 MeSH
650	_	2	\|a Radiation, Ionizing \|2 MeSH
650	_	2	\|a Cells, Cultured \|2 MeSH
650	_	2	\|a DNA Damage \|2 MeSH
693	_	_	\|a HTA \|e SESA: ESA Experiments at SIS (POF4-633; HTA) \|1 EXP:(DE-Ds200)HTA-20200803 \|0 EXP:(DE-Ds200)SESA-20200803 \|5 EXP:(DE-Ds200)SESA-20200803 \|x 0
700	1	_	\|a Parisi, Alessio \|0 P:(DE-HGF)0 \|b 1
700	1	_	\|a Miranda, Silvana \|0 P:(DE-HGF)0 \|b 2
700	1	_	\|a Gautam, Kiran \|0 P:(DE-HGF)0 \|b 3
700	1	_	\|a Vermeesen, Randy \|0 P:(DE-HGF)0 \|b 4
700	1	_	\|a Rehnberg, Emil \|0 P:(DE-HGF)0 \|b 5
700	1	_	\|a Tabury, Kevin \|0 P:(DE-HGF)0 \|b 6
700	1	_	\|a Coppes, Rob \|0 P:(DE-HGF)0 \|b 7
700	1	_	\|a van Goethem, Marc-Jan \|0 P:(DE-HGF)0 \|b 8
700	1	_	\|a Brandenburg, Sytze \|0 P:(DE-HGF)0 \|b 9
700	1	_	\|a Weber, Ulrich \|0 P:(DE-Ds200)OR2545 \|b 10
700	1	_	\|a Fournier, Claudia \|0 P:(DE-Ds200)OR0311 \|b 11
700	1	_	\|a Durante, Marco \|0 P:(DE-Ds200)OR2413 \|b 12
700	1	_	\|a Baselet, Bjorn \|0 P:(DE-HGF)0 \|b 13 \|e Corresponding author
700	1	_	\|a Baatout, Sarah \|0 P:(DE-HGF)0 \|b 14
773	_	_	\|a 10.1038/s41598-024-60912-y \|g Vol. 14, no. 1, p. 10400 \|0 PERI:(DE-600)2615211-3 \|n 1 \|p 10400 \|t Scientific reports \|v 14 \|y 2024 \|x 2045-2322
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Marc 21

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