354897 20250717224053.0 10.1038/s41598-024-60912-y doi 10.15120/GSI-2025-00017 datacite_doi altmetric:163173501 altmetric pmid:38710823 pmid WOS:001253949600063 WOS GSI-2025-00017 English 600 Radstake, Wilhelmina E. P:(DE-HGF)0 0 Radiation-induced DNA double-strand breaks in cortisol exposed fibroblasts as quantified with the novel foci-integrated damage complexity score (FIDCS) [London] 2024 Macmillan Publishers Limited, part of Springer Nature article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1752758621_898617 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote Grant: SA-IBER (AO-2019-IBER) " This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/." Without the protective shielding of Earth's atmosphere, astronauts face higher doses of ionizing radiation in space, causing serious health concerns. Highly charged and high energy (HZE) particles are particularly effective in causing complex and difficult-to-repair DNA double-strand breaks compared to low linear energy transfer. Additionally, chronic cortisol exposure during spaceflight raises further concerns, although its specific impact on DNA damage and repair remains unknown. This study explorers the effect of different radiation qualities (photons, protons, carbon, and iron ions) on the DNA damage and repair of cortisol-conditioned primary human dermal fibroblasts. Besides, we introduce a new measure, the Foci-Integrated Damage Complexity Score (FIDCS), to assess DNA damage complexity by analyzing focus area and fluorescent intensity. Our results show that the FIDCS captured the DNA damage induced by different radiation qualities better than counting the number of foci, as traditionally done. Besides, using this measure, we were able to identify differences in DNA damage between cortisol-exposed cells and controls. This suggests that, besides measuring the total number of foci, considering the complexity of the DNA damage by means of the FIDCS can provide additional and, in our case, improved information when comparing different radiation qualities. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) G:(DE-HGF)POF4-633 POF4-633 POF IV 0 FAIR Phase-0 - FAIR Phase-0 Research Program (GSI-FAIR-Phase-0) G:(Ds200)GSI-FAIR-Phase-0 GSI-FAIR-Phase-0 1 SUC-GSI-Darmstadt - Strategic university cooperation GSI-TU Darmstadt (SUC-GSI-DA) G:(DE-Ds200)SUC-GSI-DA SUC-GSI-DA 2 Dataset connected to CrossRef, Journals: repository.gsi.de Carbon ions Other Cortisol Other DNA damage Other DNA repair Other Fibroblast Other Ionizing radiation Other Iron ions Other Protons Other Hydrocortisone WI4X0X7BPJ NLM Chemicals Humans MeSH Fibroblasts: radiation effects MeSH Fibroblasts: metabolism MeSH DNA Breaks, Double-Stranded: radiation effects MeSH Hydrocortisone: pharmacology MeSH DNA Repair MeSH Radiation, Ionizing MeSH Cells, Cultured MeSH DNA Damage MeSH HTA SESA: ESA Experiments at SIS (POF4-633; HTA) EXP:(DE-Ds200)HTA-20200803 EXP:(DE-Ds200)SESA-20200803 EXP:(DE-Ds200)SESA-20200803 0 Parisi, Alessio P:(DE-HGF)0 1 Miranda, Silvana P:(DE-HGF)0 2 Gautam, Kiran P:(DE-HGF)0 3 Vermeesen, Randy P:(DE-HGF)0 4 Rehnberg, Emil P:(DE-HGF)0 5 Tabury, Kevin P:(DE-HGF)0 6 Coppes, Rob P:(DE-HGF)0 7 van Goethem, Marc-Jan P:(DE-HGF)0 8 Brandenburg, Sytze P:(DE-HGF)0 9 Weber, Ulrich P:(DE-Ds200)OR2545 10 Fournier, Claudia P:(DE-Ds200)OR0311 11 Durante, Marco P:(DE-Ds200)OR2413 12 Baselet, Bjorn P:(DE-HGF)0 13 Corresponding author Baatout, Sarah P:(DE-HGF)0 14 10.1038/s41598-024-60912-y Vol. 14, no. 1, p. 10400 PERI:(DE-600)2615211-3 1 10400 Scientific reports 14 2024 2045-2322 http://repository.gsi.de/record/354897/files/s41598-024-60912-y.pdf OpenAccess http://repository.gsi.de/record/354897/files/s41598-024-60912-y.pdf?subformat=pdfa pdfa OpenAccess oai:repository.gsi.de:354897 openaire open_access VDB driver dnbdelivery GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 10 P:(DE-Ds200)OR2545 GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 11 P:(DE-Ds200)OR0311 GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 12 P:(DE-Ds200)OR2413 DE-HGF Forschungsbereich Materie Von Materie zu Materialien und Leben G:(DE-HGF)POF4-630 G:(DE-HGF)POF4-633 G:(DE-HGF)POF4 G:(DE-HGF)POF4-600 G:(DE-HGF)POF Life Sciences – Building Blocks of Life: Structure and Function 0 2024 Creative Commons Attribution CC BY 4.0 LIC:(DE-HGF)CCBY4 HGFVOC DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2023-08-24 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2023-08-24 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2023-08-24 DBCoverage StatID:(DE-HGF)1190 StatID Biological Abstracts 2023-08-24 DBCoverage StatID:(DE-HGF)0600 StatID Ebsco Academic Search 2023-08-24 DBCoverage StatID:(DE-HGF)1040 StatID Zoological Record 2023-08-24 JCR StatID:(DE-HGF)0100 StatID SCI REP-UK : 2022 2023-08-24 DBCoverage StatID:(DE-HGF)0501 StatID DOAJ Seal 2023-04-12T15:11:06Z DBCoverage StatID:(DE-HGF)0500 StatID DOAJ 2023-04-12T15:11:06Z WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2023-08-24 Fees StatID:(DE-HGF)0700 StatID 2023-08-24 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2023-08-24 IF < 5 StatID:(DE-HGF)9900 StatID 2023-08-24 OpenAccess StatID:(DE-HGF)0510 StatID Peer Review StatID:(DE-HGF)0030 StatID ASC 2023-08-24 Article Processing Charges StatID:(DE-HGF)0561 StatID 2023-08-24 DBCoverage StatID:(DE-HGF)1150 StatID Current Contents - Physical, Chemical and Earth Sciences 2023-08-24 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2023-08-24 DBCoverage StatID:(DE-HGF)0320 StatID PubMed Central 2023-08-24 DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2023-08-24 yes I:(DE-Ds200)BIO-20160831OR354 BIO Biophysik 0 I:(DE-Ds200)Coll-FAIR-BIO BIO@FAIR Collaboration FAIR: BIO 1 journal VDB I:(DE-Ds200)BIO-20160831OR354 I:(DE-Ds200)Coll-FAIR-BIO UNRESTRICTED FullTexts