359584 20260304232653.0 10.1016/j.adro.2025.101783 doi 10.15120/GSI-2025-00765 datacite_doi 40486289 pmid GSI-2025-00765 English 610 Totis, Cristina P:(DE-Ds200)OR10627 0 First author Induction of Cytoplasmic dsDNA and cGAS-STING Immune Signaling After Exposure of Breast Cancer Cells to X-ray or High-Energetic Carbon Ions Amsterdam 2025 Elsevier article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1772637724_332469 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Radiation therapy can trigger activation of the cyclic GMP-AMP synthase (cGAS)- Stimulator of interferon genes (STING) axis via cytoplasmic dsDNA fragment induction. The activation of cGAS-STING initiates innate immune signaling mediated by interferon type I that can contribute to eradicate the malignancy. The effect was shown to depend on the fractionation scheme employed. We hypothesized that the innate immune response can also depend on radiation quality because densely ionizing radiation, such as carbon ions, have different effects on DNA lesion quality.We exposed an in vitro 4T1 breast cancer model to either photons or carbon ions and measured the clonogenic survival of cells with the colony-forming assay. The occurrence of cytosolic dsDNA fragments was assessed via immunofluorescence, whereas the expression and release of interferon-β by quantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Bulk RNA sequencing was used to investigate global radiation-induced changes in gene expression.We show here that carbon ions induced a significantly higher yield of cytosolic dsDNA fragments per unit dose as compared to photons. The higher efficiency also translated in expression and release of interferon-β by the tumor cells. The rate of cytoplasmic dsDNA foci as well as interferon-β release increased with doses up to 20 Gy and no differences for a fractionation scheme (3 × 8 Gy) were found as compared to the single high doses (20 or 24 Gy) of photons.In conclusion, we found that the release of interferon-β after radiation increases with the radiation dose up to 20 Gy and that carbon ions have the potential to elicit a strong innate immune signaling. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) G:(DE-HGF)POF4-633 POF4-633 POF IV 0 FAIR Phase-0 - FAIR Phase-0 Research Program (GSI-FAIR-Phase-0) G:(Ds200)GSI-FAIR-Phase-0 GSI-FAIR-Phase-0 1 SUC-GSI-Darmstadt - Strategic university cooperation GSI-TU Darmstadt (SUC-GSI-DA) G:(DE-Ds200)SUC-GSI-DA SUC-GSI-DA 2 50WB2014 - Molekulare Grundlagen der Krebsentstehung durch kosmische Strahlung (BMWK-50WB2014) G:(DE-Ds200)BMWK-50WB2014 BMWK-50WB2014 3 02NUK054A - Verbundprojekt VERCHROMT II: Erkennung, Verarbeitung und biologische Konsequenzen von Chromatinschäden nach Teilchenbestrahlung II, Teilprojekt A (BMBF-02NUK054A) G:(DE-Ds200)BMBF-02NUK054A BMBF-02NUK054A 4 BMBF-02NUK050A - Genetische Risiken und entzündungshemmende Wirkung von dicht-ionisierender alpha-Strahlung - Teilprojekt A (BMBF-02NUK050A) G:(DE-Ds200)BMBF-02NUK050A BMBF-02NUK050A 5 Dataset connected to CrossRef, Journals: repository.gsi.de HTA SBio_Jakob: Radiation-induced DNA damage responses in the context of LET, chromatin, and cell cycle (POF3-315; HTA) EXP:(DE-Ds200)HTA-20200803 EXP:(DE-Ds200)SBio_Jakob-20200803 EXP:(DE-Ds200)SBio_Jakob-20200803 0 HTA SBio_Fournier: Modulation of immune and inflammatory response by densely ionizing irradiation (POF3-315; HTA) EXP:(DE-Ds200)HTA-20200803 EXP:(DE-Ds200)SBio_Fournier-20200803 EXP:(DE-Ds200)SBio_Fournier-20200803 1 Averbeck, Nicole B. 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P:(DE-Ds200)OR11088 5 Durante, Marco P:(DE-Ds200)OR2413 6 Fournier, Claudia P:(DE-Ds200)OR0311 7 Helm, Alexander P:(DE-Ds200)OR3001 8 Corresponding author 10.1016/j.adro.2025.101783 Vol. 10, no. 6, p. 101783 - PERI:(DE-600)2847724-8 6 101783 Advances in radiation oncology 10 2025 2452-1094 http://repository.gsi.de/record/359584/files/1-s2.0-S2452109425000703-main.pdf OpenAccess http://repository.gsi.de/record/359584/files/1-s2.0-S2452109425000703-main.pdf?subformat=pdfa pdfa OpenAccess oai:repository.gsi.de:359584 openaire open_access OpenAPC driver VDB openCost dnbdelivery GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 0 P:(DE-Ds200)OR10627 GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 1 P:(DE-Ds200)OR2525 GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 2 P:(DE-Ds200)OR0522 GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 3 P:(DE-Ds200)OR9110 GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 4 P:(DE-Ds200)OR11433 GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 5 P:(DE-Ds200)OR11088 GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 6 P:(DE-Ds200)OR2413 GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 7 P:(DE-Ds200)OR0311 GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 8 P:(DE-Ds200)OR3001 DE-HGF Forschungsbereich Materie Von Materie zu Materialien und Leben G:(DE-HGF)POF4-630 G:(DE-HGF)POF4-633 G:(DE-HGF)POF4 G:(DE-HGF)POF4-600 G:(DE-HGF)POF Life Sciences – Building Blocks of Life: Structure and Function 0 2025 APC keys set PC:(DE-HGF)0000 APC Local Funding PC:(DE-HGF)0001 APC DFG OA Publikationskosten PC:(DE-HGF)0002 APC DOAJ Journal PC:(DE-HGF)0003 APC Creative Commons Attribution CC BY 4.0 LIC:(DE-HGF)CCBY4 HGFVOC OpenAccess StatID:(DE-HGF)0510 StatID JCR StatID:(DE-HGF)0100 StatID ADV RADIAT ONCOL : 2022 2024-12-12 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2024-12-12 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2024-12-12 DBCoverage StatID:(DE-HGF)0501 StatID DOAJ Seal 2023-05-02T08:52:39Z DBCoverage StatID:(DE-HGF)0500 StatID DOAJ 2023-05-02T08:52:39Z Peer Review StatID:(DE-HGF)0030 StatID DOAJ : Double anonymous peer review 2023-05-02T08:52:39Z DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2024-12-12 WoS StatID:(DE-HGF)0112 StatID Emerging Sources Citation Index 2024-12-12 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2024-12-12 IF < 5 StatID:(DE-HGF)9900 StatID 2024-12-12 Article Processing Charges StatID:(DE-HGF)0561 StatID 2024-12-12 Fees StatID:(DE-HGF)0700 StatID 2024-12-12 yes I:(DE-Ds200)BIO-20160831OR354 BIO Biophysik 0 I:(DE-Ds200)Coll-FAIR-BIO BIO@FAIR Collaboration FAIR: BIO 1 journal VDB I:(DE-Ds200)BIO-20160831OR354 I:(DE-Ds200)Coll-FAIR-BIO APC UNRESTRICTED APC FullTexts