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@ARTICLE{Benje:362975,
      author       = {Benje, Malte and Vitacchio, Tamara and Fritsche, Dennis and
                      Tinganelli, Walter},
      title        = {{G}ene {E}xpression {P}rofiling and {P}henotypic
                      {C}haracterization of {C}irculating {T}umor {C}ells
                      {D}erived from a {M}urine {O}steosarcoma {M}odel},
      journal      = {Cancers},
      volume       = {17},
      number       = {7},
      issn         = {2072-6694},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {GSI-2025-01218},
      pages        = {1210},
      year         = {2025},
      note         = {This article is an open access article distributed under
                      the terms and conditions of the Creative Commons Attribution
                      (CC BY) license
                      (https://creativecommons.org/licenses/by/4.0/).},
      abstract     = {Osteosarcoma is an aggressive bone malignancy with high
                      metastatic potential to the lungs. CTCs, as seeds of
                      metastasis, play an important role in the spread of this
                      cancer, and, therefore, their isolation, culture, and gene
                      expression analysis promises valuable insights into the
                      progression and metastatic cascade of osteosarcoma. The aim
                      of this study was to isolate and culture CTCs from
                      osteosarcoma-bearing mice and compare their migration,
                      radioresistance, and gene expression with their parental
                      cell line.CTCs from LM8-inoculated mice were isolated and
                      cultured. The gene expression of the CTC-derived cell lines
                      was then compared to the parental cell line. Furthermore, a
                      Transwell assay, a clonogenic assay after irradiation, and
                      immunohistochemical stainings were used to compare the
                      CTC-derived cell lines with the parental cell line.The
                      CTC-derived cell lines differed significantly in gene
                      expression from their parental cell line. 361 differentially
                      expressed genes were identified, among which GO and KEGG
                      analysis revealed major differences in the expression of
                      genes related to antigen processing and presentation and
                      extracellular matrix constituents. In addition, the
                      CTC-derived cell lines were observed to have a higher
                      migratory capacity and comparable radioresistance compared
                      to the parental cell line. CD44 expression was found to be
                      conserved in CTC-derived cell lines.This study provides a
                      comparison between CTC-derived and their parental cell lines
                      in terms of gene expression, migration, and radioresistance.
                      Our findings allow for further research in the field of
                      osteosarcoma CTCs and their generation. Furthermore, the
                      identified DEGs between CTCs and their parental cell line
                      can serve as a reference point for targeted therapies
                      against osteosarcoma CTCs.},
      keywords     = {circulating tumor cells (Other) / mRNA-seq (Other) /
                      osteosarcoma (Other)},
      cin          = {BIO},
      ddc          = {610},
      cid          = {I:(DE-Ds200)BIO-20160831OR354},
      pnm          = {633 - Life Sciences – Building Blocks of Life: Structure
                      and Function (POF4-633)},
      pid          = {G:(DE-HGF)POF4-633},
      experiment   = {$EXP:(DE-Ds200)no_experiment-20200803$},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.3390/cancers17071210},
      url          = {https://repository.gsi.de/record/362975},
}