362975 20260303232643.0 10.3390/cancers17071210 doi 10.15120/GSI-2025-01218 datacite_doi pmid:40227761 pmid WOS:001463614200001 WOS GSI-2025-01218 English 610 Benje, Malte P:(DE-Ds200)OR12987 0 First author gsi Gene Expression Profiling and Phenotypic Characterization of Circulating Tumor Cells Derived from a Murine Osteosarcoma Model Basel 2025 MDPI article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1772543322_2575950 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Osteosarcoma is an aggressive bone malignancy with high metastatic potential to the lungs. CTCs, as seeds of metastasis, play an important role in the spread of this cancer, and, therefore, their isolation, culture, and gene expression analysis promises valuable insights into the progression and metastatic cascade of osteosarcoma. The aim of this study was to isolate and culture CTCs from osteosarcoma-bearing mice and compare their migration, radioresistance, and gene expression with their parental cell line.CTCs from LM8-inoculated mice were isolated and cultured. The gene expression of the CTC-derived cell lines was then compared to the parental cell line. Furthermore, a Transwell assay, a clonogenic assay after irradiation, and immunohistochemical stainings were used to compare the CTC-derived cell lines with the parental cell line.The CTC-derived cell lines differed significantly in gene expression from their parental cell line. 361 differentially expressed genes were identified, among which GO and KEGG analysis revealed major differences in the expression of genes related to antigen processing and presentation and extracellular matrix constituents. In addition, the CTC-derived cell lines were observed to have a higher migratory capacity and comparable radioresistance compared to the parental cell line. CD44 expression was found to be conserved in CTC-derived cell lines.This study provides a comparison between CTC-derived and their parental cell lines in terms of gene expression, migration, and radioresistance. Our findings allow for further research in the field of osteosarcoma CTCs and their generation. Furthermore, the identified DEGs between CTCs and their parental cell line can serve as a reference point for targeted therapies against osteosarcoma CTCs. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) G:(DE-HGF)POF4-633 POF4-633 POF IV 0 Dataset connected to CrossRef, Journals: repository.gsi.de circulating tumor cells Other mRNA-seq Other osteosarcoma Other theory no experiment theory work (theory) EXP:(DE-Ds200)theory-20200803 EXP:(DE-Ds200)no_experiment-20200803 EXP:(DE-Ds200)no_experiment-20200803 0 Vitacchio, Tamara P:(DE-Ds200)OR11493 1 gsi Fritsche, Dennis P:(DE-HGF)0 2 Tinganelli, Walter P:(DE-Ds200)OR2924 3 Corresponding author gsi 10.3390/cancers17071210 Vol. 17, no. 7, p. 1210 - PERI:(DE-600)2527080-1 7 1210 Cancers 17 2025 2072-6694 http://repository.gsi.de/record/362975/files/cancers-17-01210.pdf OpenAccess http://repository.gsi.de/record/362975/files/cancers-17-01210.pdf?subformat=pdfa pdfa OpenAccess oai:repository.gsi.de:362975 openaire open_access VDB driver dnbdelivery GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 0 P:(DE-Ds200)OR12987 GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 1 P:(DE-Ds200)OR11493 GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 2 P:(DE-HGF)0 GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 3 P:(DE-Ds200)OR2924 DE-HGF Forschungsbereich Materie Von Materie zu Materialien und Leben G:(DE-HGF)POF4-630 G:(DE-HGF)POF4-633 G:(DE-HGF)POF4 G:(DE-HGF)POF4-600 G:(DE-HGF)POF Life Sciences – Building Blocks of Life: Structure and Function 0 2025 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2024-12-14 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2024-12-14 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2024-12-14 DBCoverage StatID:(DE-HGF)1190 StatID Biological Abstracts 2024-12-14 DBCoverage StatID:(DE-HGF)0600 StatID Ebsco Academic Search 2024-12-14 JCR StatID:(DE-HGF)0100 StatID CANCERS : 2022 2024-12-14 WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2024-12-14 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2024-12-14 OpenAccess StatID:(DE-HGF)0510 StatID Peer Review StatID:(DE-HGF)0030 StatID ASC 2024-12-14 IF >= 5 StatID:(DE-HGF)9905 StatID CANCERS : 2022 2024-12-14 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2024-12-14 Creative Commons Attribution CC BY 4.0 LIC:(DE-HGF)CCBY4 HGFVOC DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2024-12-14 yes I:(DE-Ds200)BIO-20160831OR354 BIO Biophysik 0 I:(DE-Ds200)Coll-FAIR-BIO BIO@FAIR Collaboration FAIR: BIO 1 journal VDB I:(DE-Ds200)BIO-20160831OR354 I:(DE-Ds200)Coll-FAIR-BIO UNRESTRICTED FullTexts