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000366781 0247_ $$2datacite_doi$$a10.15120/GSI-2026-00570
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000366781 1001_ $$0P:(DE-HGF)0$$aFatmaoui, Fadwa$$b0
000366781 245__ $$aDirect visualization and tracing of chromatin folding in the Drosophila embryo
000366781 260__ $$a[London]$$bNature Publishing Group UK$$c2026
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000366781 520__ $$aChromatin organization, through the assembly of DNA with histones and the folding of nucleosome chains, regulates DNA accessibility for transcription, DNA replication and repair. Although models derived from in vitro studies have proposed distinct nucleosome chain geometries, the organization of chromatin within the crowded cell nucleus remains elusive. Using cryo-electron tomography of thin vitreous sections, we directly observed the path of nucleosomal and linker DNA in situ from a flash-frozen organism - Drosophila embryos. We quantified linker length and curvature, characterizing an irregular zig-zag chromatin-folding motif, with a low degree of linker bending. Nucleosome conformations could be identified on individual particles in favorable orientations without structure averaging. Additionally, we observed particles that accommodate a number of DNA gyres ranging from less than one to up to three, which resemble previously proposed non-octameric nucleosomal particles with variable DNA wrapping.
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000366781 536__ $$0G:(DE-Ds200)BMBF-02NUK054A$$a02NUK054A - Verbundprojekt VERCHROMT II: Erkennung, Verarbeitung und biologische Konsequenzen von Chromatinschäden nach Teilchenbestrahlung II, Teilprojekt A (BMBF-02NUK054A)$$cBMBF-02NUK054A$$x2
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000366781 650_7 $$2Other$$aChromatin Fiber
000366781 650_7 $$2Other$$aIn Situ Cryo-Electron Tomography
000366781 650_7 $$2Other$$aLinker DNA
000366781 650_7 $$2Other$$aNucleosome
000366781 650_7 $$2Other$$aSub-Nucleosomal Particles
000366781 650_7 $$2NLM Chemicals$$aNucleosomes
000366781 650_7 $$2NLM Chemicals$$aChromatin
000366781 650_7 $$09007-49-2$$2NLM Chemicals$$aDNA
000366781 650_2 $$2MeSH$$aAnimals
000366781 650_2 $$2MeSH$$aNucleosomes: ultrastructure
000366781 650_2 $$2MeSH$$aNucleosomes: metabolism
000366781 650_2 $$2MeSH$$aChromatin: ultrastructure
000366781 650_2 $$2MeSH$$aChromatin: metabolism
000366781 650_2 $$2MeSH$$aChromatin: chemistry
000366781 650_2 $$2MeSH$$aEmbryo, Nonmammalian: ultrastructure
000366781 650_2 $$2MeSH$$aEmbryo, Nonmammalian: metabolism
000366781 650_2 $$2MeSH$$aDrosophila melanogaster: embryology
000366781 650_2 $$2MeSH$$aDNA: metabolism
000366781 650_2 $$2MeSH$$aDNA: chemistry
000366781 650_2 $$2MeSH$$aDNA: ultrastructure
000366781 650_2 $$2MeSH$$aCryoelectron Microscopy
000366781 650_2 $$2MeSH$$aElectron Microscope Tomography
000366781 650_2 $$2MeSH$$aDrosophila: embryology
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000366781 7001_ $$0P:(DE-HGF)0$$aCarrivain, Pascal$$b1
000366781 7001_ $$0P:(DE-HGF)0$$aTaiki, Fatima$$b2
000366781 7001_ $$00009-0001-5919-7907$$aIusupova, Amina$$b3
000366781 7001_ $$0P:(DE-HGF)0$$aGrewe, Diana$$b4
000366781 7001_ $$00000-0001-6229-2692$$aHagen, Wim$$b5
000366781 7001_ $$0P:(DE-Ds200)OR0522$$aJakob, Burkhard$$b6$$ugsi
000366781 7001_ $$00000-0002-3815-8896$$aVictor, Jean-Marc$$b7$$eCorresponding author
000366781 7001_ $$00000-0002-0225-2776$$aLeforestier, Amélie$$b8$$eCorresponding author
000366781 7001_ $$00000-0003-2298-8964$$aEltsov, Mikhail$$b9$$eCorresponding author
000366781 773__ $$0PERI:(DE-600)1467419-1$$a10.1038/s44318-026-00701-7$$gVol. 45, no. 5, p. 1621 - 1647$$n5$$p1621 - 1647$$tThe EMBO journal$$v45$$x0261-4189$$y2026
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