Hauptseite > Publikationsdatenbank > Direct visualization and tracing of chromatin folding in the Drosophila embryo > print

001     366781
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024 7 _ |a 10.1038/s44318-026-00701-7
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024 7 _ |a 10.15120/GSI-2026-00570
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100 1 _ |a Fatmaoui, Fadwa
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245 _ _ |a Direct visualization and tracing of chromatin folding in the Drosophila embryo
260 _ _ |a [London]
|c 2026
|b Nature Publishing Group UK
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500 _ _ |a This article is licensed under a Creative Commons Attribution 4.0 International License
520 _ _ |a Chromatin organization, through the assembly of DNA with histones and the folding of nucleosome chains, regulates DNA accessibility for transcription, DNA replication and repair. Although models derived from in vitro studies have proposed distinct nucleosome chain geometries, the organization of chromatin within the crowded cell nucleus remains elusive. Using cryo-electron tomography of thin vitreous sections, we directly observed the path of nucleosomal and linker DNA in situ from a flash-frozen organism - Drosophila embryos. We quantified linker length and curvature, characterizing an irregular zig-zag chromatin-folding motif, with a low degree of linker bending. Nucleosome conformations could be identified on individual particles in favorable orientations without structure averaging. Additionally, we observed particles that accommodate a number of DNA gyres ranging from less than one to up to three, which resemble previously proposed non-octameric nucleosomal particles with variable DNA wrapping.
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536 _ _ |a SUC-GSI-Darmstadt - Strategic university cooperation GSI-TU Darmstadt (SUC-GSI-DA)
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536 _ _ |a 02NUK054A - Verbundprojekt VERCHROMT II: Erkennung, Verarbeitung und biologische Konsequenzen von Chromatinschäden nach Teilchenbestrahlung II, Teilprojekt A (BMBF-02NUK054A)
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588 _ _ |a Dataset connected to DataCite
650 _ 7 |a Chromatin Fiber
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650 _ 7 |a In Situ Cryo-Electron Tomography
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650 _ 7 |a Linker DNA
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650 _ 7 |a Nucleosome
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650 _ 7 |a Sub-Nucleosomal Particles
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650 _ 7 |a Nucleosomes
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650 _ 7 |a Chromatin
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650 _ 7 |a DNA
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650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Nucleosomes: ultrastructure
|2 MeSH
650 _ 2 |a Nucleosomes: metabolism
|2 MeSH
650 _ 2 |a Chromatin: ultrastructure
|2 MeSH
650 _ 2 |a Chromatin: metabolism
|2 MeSH
650 _ 2 |a Chromatin: chemistry
|2 MeSH
650 _ 2 |a Embryo, Nonmammalian: ultrastructure
|2 MeSH
650 _ 2 |a Embryo, Nonmammalian: metabolism
|2 MeSH
650 _ 2 |a Drosophila melanogaster: embryology
|2 MeSH
650 _ 2 |a DNA: metabolism
|2 MeSH
650 _ 2 |a DNA: chemistry
|2 MeSH
650 _ 2 |a DNA: ultrastructure
|2 MeSH
650 _ 2 |a Cryoelectron Microscopy
|2 MeSH
650 _ 2 |a Electron Microscope Tomography
|2 MeSH
650 _ 2 |a Drosophila: embryology
|2 MeSH
693 _ _ |a theory
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700 1 _ |a Carrivain, Pascal
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700 1 _ |a Taiki, Fatima
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700 1 _ |a Iusupova, Amina
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700 1 _ |a Grewe, Diana
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700 1 _ |a Hagen, Wim
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700 1 _ |a Jakob, Burkhard
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700 1 _ |a Victor, Jean-Marc
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700 1 _ |a Leforestier, Amélie
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700 1 _ |a Eltsov, Mikhail
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773 _ _ |a 10.1038/s44318-026-00701-7
|g Vol. 45, no. 5, p. 1621 - 1647
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