368177 20260602221827.0 10.1002/smll.202504070 doi 1613-6810 ISSN 1613-6829 ISSN 10.15120/GSI-2026-00734 datacite_doi GSI-2026-00734 English 620 Küstner, Merle-Johannaex P:(DE-HGF)0 0 Biotechnical Multiscale Engineering of Scaffolds for Stem Cell and Organoid Research Weinheim 2026 Wiley-VCH article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1780386269_3617093 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote This is an open access article under the terms of the Creative Commons Attribution License 4. The publication describes complex support structures and scaffolds for stem cells and organoids. Consideration of geometric and structural parameters has an influence on stem cell development and organogenesis comparable to that of molecular genetics and biochemical parameters. Two essential representatives are discussed here in more detail: hematopoietic stem cells (HSCs) and brain organoids. Due to their ability to fully regenerate the blood system, HSCs are used for stem cell transplantations. Therefore, efficient approaches to create an artificial but close-to-nature stem cell niche in vitro for amplification of HSCs are highly desirable. Apart from biochemical and biological factors, geometrical and biomechanical parameters are important. Biotechnological multiscale engineering is able to mimic the HSC niche, improving their amplification. Another highly dynamic process underlying hierarchic orders is organogenesis. Entire organs develop from individual cells that are spatially arranged in precise patterns and exposed to chemical, mechanical, and structural stimuli. The significance of the different scales during their development is explained using human brain organoids. Here, geometrically suitable structures improve biochemical differentiation protocols. Such technical hybrid systems can foster research of a rather inaccessible organ and possibly serve as a platform for more energy-efficient computing devices, such as organoid automata, hence, orgamats. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) G:(DE-HGF)POF4-633 POF4-633 POF IV 0 Dataset connected to CrossRef, Journals: repository.gsi.de biophysical observation models Other biotechnical multiscale engineering Other brain organoids Other hematopoietic stemcells Other micro/nanotechnology Other orgamats Other scaffolds Other Organoids: cytology MeSH Humans MeSH Tissue Scaffolds: chemistry MeSH Tissue Engineering: methods MeSH Stem Cells: cytology MeSH Biotechnology: methods MeSH Animals MeSH Cell Differentiation MeSH Hematopoietic Stem Cells: cytology MeSH none Biophysics experiment at GSI, but no LSRF/no experiment at the GSI accelerator complex (none) EXP:(DE-Ds200)none-20200803 EXP:(DE-Ds200)Biophysics_no_LSRF/EXP-20200803 EXP:(DE-Ds200)Biophysics_no_LSRF/EXP-20200803 0 Marx, Christian P:(DE-HGF)0 1 Graben, Peter beim P:(DE-HGF)0 2 Rodrigues, Serafim P:(DE-HGF)0 3 Hampl, Jörg P:(DE-HGF)0 4 Weise, Frank P:(DE-HGF)0 5 Gebinoga, Michael P:(DE-HGF)0 6 Kaysan, Leon P:(DE-Ds200)OR12783 7 gsi Klett, Maren P:(DE-HGF)0 8 Brauer, Dana P:(DE-HGF)0 9 Schlingloff, Gregor P:(DE-HGF)0 10 Heinrich, Doris P:(DE-HGF)0 11 Schroeder, Insa Sigrid P:(DE-Ds200)OR6462 12 gsi Schober, Andreas P:(DE-HGF)0 13 Corresponding author 10.1002/smll.202504070 Vol. 22, no. 12, p. e04070 PERI:(DE-600)2168935-0 12 e04070 Small 22 2026 1613-6810 OpenAccess http://repository.gsi.de/record/368177/files/Small%20-%202025%20-%20K%C3%BCstner%20-%20Biotechnical%20Multiscale%20Engineering%20of%20Scaffolds%20for%20Stem%20Cell%20and%20Organoid%20Research.pdf OpenAccess pdfa http://repository.gsi.de/record/368177/files/Small%20-%202025%20-%20K%C3%BCstner%20-%20Biotechnical%20Multiscale%20Engineering%20of%20Scaffolds%20for%20Stem%20Cell%20and%20Organoid%20Research.pdf?subformat=pdfa oai:repository.gsi.de:368177 openaire open_access VDB driver dnbdelivery GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 7 P:(DE-Ds200)OR12783 GSI Helmholtzzentrum für Schwerionenforschung GmbH I:(DE-Ds200)20121206GSI GSI 12 P:(DE-Ds200)OR6462 DE-HGF Forschungsbereich Materie Von Materie zu Materialien und Leben G:(DE-HGF)POF4-630 G:(DE-HGF)POF4-633 G:(DE-HGF)POF4 G:(DE-HGF)POF4-600 G:(DE-HGF)POF Life Sciences – Building Blocks of Life: Structure and Function 0 2026 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2025-11-05 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2025-11-05 Creative Commons Attribution CC BY 4.0 LIC:(DE-HGF)CCBY4 HGFVOC DBCoverage StatID:(DE-HGF)1150 StatID Current Contents - Physical, Chemical and Earth Sciences 2025-11-05 DEAL Wiley StatID:(DE-HGF)3001 StatID 2025-11-05 ger WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2025-11-05 IF >= 10 StatID:(DE-HGF)9910 StatID SMALL : 2022 2025-11-05 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2025-11-05 OpenAccess StatID:(DE-HGF)0510 StatID JCR StatID:(DE-HGF)0100 StatID SMALL : 2022 2025-11-05 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2025-11-05 DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2025-11-05 yes I:(DE-Ds200)BIO-20160831OR354 BIO Biophysik 0 journal VDB UNRESTRICTED I:(DE-Ds200)BIO-20160831OR354 FullTexts